Melbourne's Crown Casino has been hit with more allegations of poker machine tampering, with federal independent MP Andrew Wilkie claiming he has the "smoking gun" to back them up.

Mr Wilkie on Tuesday showed journalists chips he alleges were given to a former poker99 high roller at the casino, allowing her to tamper with machines and play more than one at a time.

The Hobart-based politician says he had met with Victoria's gambling regulator and written to police about the claims, which he added could be the "smoking gun" proving Crown broke the law.

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